Symbiont Abundance Notebook - University of Washington - SAFS - Padilla-Gamiño Lab

qPCR - CAct Primer Test With 10ng Input DNA

2023

qPCR

CFX Connect

CAct

Previous CAct qPCRs from 20231210 (run 1 and run 2) exhibited lack of amplification in 100pg and 10pg input DNA quantities, as well as poor replication in 1000pg DNA input quantity, so I decided to see if poor replication…

qPCR - Repeat CAct Primer Test

2023

CFX Connect

qPCR

CAct

Due to odd results from earlier today (Notebook), I repeated this qPCR to see if I could get better replication and consistent melt curves across samples.

qPCR - New CAct Primer Test

CAct

qPCR

CFX Connect

2023

Previous qPCR from 20231119 had poor results with the CAct primer set (C symbiont). No amplication at 1000pg, a single sample amplified at 100pg, and no amplification at 10pg of input…

qPCR - Initial Primer Tests with Positive Control Serial Dilutions

qPCR

CFX Connect

2023

After quantifying the DNA and awaiting a response on how to handle the lack of DNA in 20 of…

DNA Quantification - Initial DNA Assessment Using Qubit hsDNA Assay

2023

Qubit

hsDNA

DNA quantification

To begin this project, I opted to re-quantify all of the DNA samples provided using the Qubit high senstivity DNA Assay (hsDNA; Invitrogen). I believe the samples had previously been quantified using a NanoDrop. However, looking at the values, many…